Instrumentation in the facility

QTOF6520 with HPLC

The Agilent 6520 Quadrupole Time-of-Flight LC/MS instrument features:

• 20-20,000 m/z range
• Attomole-level sensitivity
• Better than 2-ppm mass accuracy at MS level and 5-ppm mass accuracy at MS/MS level
• 3-4 orders of magnitude in-spectrum dynamic range enabling identification of less-abundant compounds in the presence of more-abundant compounds
• Fast MS and MS/MS spectral acquisition improving the identification of unresolved components in complex samples
• Fully Automated for overnight sample runs

AKTA Purifier

The AKTA Purifier  features the following:

• Sample injection volumes from 1 ml - 50 ml
• Flow rates up to 10ml/min and up to 25 mPa pressure
• Fully automated with fast and efficient purification
• Full range of columns available in the facility
• Compatible with some HPLC Columns
• UV-VIS, conductivity, and pH monitoring of samples

Modulus I and II Plate Readers

The Modulus II Multi-Mode Plate Reader from Turner Biosystems has the following features:

• Reads plates in 6-384 format.
• Heated Chamber from 2º to 45º C above ambient temperature .  The chamber is also configured with a factory-installed shaker that allows for either linear or orbital shaking.
• Touchscreen display for interaction. Preprogramed with all Promega Assays that are currently available that work with plate readers and has options for user defined programs. Data is easily transferred using a USB Drive and USB Stick.  All files are easily opened in EXCEL.
• A Fluorometer with detection limits of 0.5 fm/200 µl of fluorescein in 96-well plate and a linear dynamic range of 6 logs.  The fluorometer has the following detection modules: UV (Ex 365 nm, Em 410-460 nm), Blue (Ex 460 nm, Em 515-580 nm), Green (Ex 525 nm, Em 580-640 nm), and Red (Ex 625 nm, Em 660-720 nm).
• A Luminometer with a detection limit of 3 x 10-21 moles of Luciferase and a linear dynamic range of > 8 logs
• Absorbance Detection for both UV/VIS Spectrum.  UV-Visible Absorbance Detector with six-position Filter Wheel, six-installed Filters (260, 280, 450, 560, 600, 750 nm)
• The Modulus I has the same features as II except it is not temperature controlled and plates cannot be shaked

TYPHOON Trio Imager with Computer

The Typhoon™ Trio unites proven storage phosphor autoradiography technology with four color fluorescent labeling techniques for maximum data quality in a single, high-throughput system. For DNA, RNA, and protein samples, users can choose from:

• storage phosphor autoradiography
• direct green (532 nm) excited fluorescence
• direct red (633 nm) excited fluorescence
• direct blue (488 nm) excited fluorescence
• chemiluminescence

ETTAN DIGE 2D Gel Apparatus and Analysis Software

Computer  Workstations for Data Storage and Analysis

Quad Core Computer Workstations with 1TB hard drive  and 4GB of Memory with the following software/hardware packages:

• 4TB backup (Double redundancy)
• MS OFFICE 2010
• Laserjet printer
• 3in1 Printer, Scanner, and Fax
• METLIN Personal Metabolomics Database
• Spectrum Mill Proteomics Database
• IQTL (Image Quantitation for Typhoon)