Pesek, Joseph J
Professor, Chemistry
Preferred: joseph.pesek@sjsu.edu
Telephone
Preferred: (408) 924-4950
Office Hours
by appointment
PRIMARY RESEARCH INTRESTS
Students in my group explore a variety of topics. Research is divided into
two major sections:
I: Synthesis and Characterization of Novel Stationary Phases for High Performance Liquid Chromatography (HPLC)
Graduate
and undergraduate students synthesize an array of novel stationary phases using
Kromasil and AstroSil silicas as a main support. For more information on Kromasil
silica go to the web address: "www.kromasil.com/products/products/kromasil/index.htm". Information on AstroSil silica is available on the web site "www.stellarphases.com/Astrosil.htm". The picture on the left represents a scanning electron micrograph of 5 �m AstroSil
Silica (100�) at 2000x magnification. The surface of the particles is smooth and featureless,
and remains so even at much higher magnifications.
The surface of the silica used in the studies is first converted to hydride
via a silanization reaction.
The second step, hydrosilation, attaches the organic moiety to the hydride intermediate
in order to create the product that has the desired surface properties.
HYDROSILATION
cat = catalyst, typically hexachloroplatinic acid
As shown above, the bonded organic moiety is attached to the surface by a stable
Si-C bond. This feature leads to the high stability observed in chromatographic
experiments.
HPLC columns developed in my lab are being marketing by MicroSolv Inc. - see the web
site "www.microsolvtech.com/pl_hplc.asp" for more information.
II: New etched capillary columns for Open Tubular Capillary Electrophoresis
(OTCEC) - development and characterization
The open tubular approach to Capillary Electrochromatography has been the focus of work in our laboratory. The diagram below depicts the process of making modified OTCEC columns.
The finished products are then used by students in a variety of challenging
bioanalytical problems. So far the following etched capillaries have been created
and characterized: cholesterol, diol, C18, C8, butyphenyl, cyclodextrin.
Some of the columns made in my lab are available via MicroSolv Inc., see the
web site "www.microsolvtech.com/celerity_chem.asp" for more information.
Education
- Doctor of Philosophy, Univ Of Cal-Los Angeles, 1970
- Bachelor of Science, Chemistry, University of Illinois, 1966
Bio
Professional Preparation
University of Illinois Chemistry B.S. 1966
UCLA Analytical Chemistry Ph.D. 1970
UCLA Postdoctoral Fellow 1970-1971
Appointments
Professor San Jose State University 1985-
Associate Dean,
Graduate Studies and Research San Jose State University 2000-2003
Visiting Research Professor Monash University, Melbourne, Australia 2000
Chair, Dept. of Chemistry San Jose State University 1988-1996
Visiting Professor University of Aix-Marseille 1988,1992, 1994, 1995,1996
Visiting Research Professor Ecole Polytechnique, Paris 1984-1985
Associate Professor San Jose State University 1981-1985
Assistant Professor San Jose State University 1978-1981
Assistant Professor Northern Illinois University 1971-1978
Publications
Five significant publications
"Open Tubular Approaches to Capillary Electrochromatography, J. J. Pesek and M.T. Matyska, in Capillary Electrochromatography, Z. Deyl, F. Svec, eds., Elsevier, Amsterdam, 2001, pp 241-270.
"Characterization of Open Tubular Capillary Electrochromatography Columns for the analysis of Synthetic peptides using Isocratic Conditions, M.T. Matyska, J.J. Pesek, R.I. Boysen and M.T.W. Hearn, Anal. Chem., 73 (2001) 5116-5125.
"Surface Modifications to Support Materials for HPLC, HPCE and Electrochromatography, "J.J. Pesek, M.T. Matyska, in "Fundamental and Applied Aspects of Chemically Modified Surfaces, J. Blitz and C.B. Little, eds., Royal Society of Chemistry, Oxford, 1999, 97-110.
"Synthesis and Characterization of Liquid Crystal Type Stationary Phases on a Silica Hydride Surface, J.J. Pesek, M.T. Matyska, E. Williamsen, R. Tam, Z. Wang, J. Liq. Chromatogr. & Rel. Technol., 21 (1998) 2747-2762.
"Synthesis and Characterization of Bonded Phases Made Via Hydrosilation of Alkynes on Silica Hydride Surfaces, J.J. Pesek, M.T. Matyska, M. Oliva, M Evanchic, J. Chromatogr. A, 818 (1998) 145-154.
TOTAL PUBLICATIONS: 172
Synergistic Activities
Project Director - SJSU/IBM Research and Training Program supported as an NSF
GOALI initiative.
Co-PI - NSF Curriculum Development Grant, new teaching methods in quantitative
analysis.
Board of Directors, California Separation Science Society (CaSSS)
Associate Editor, Journal of Separation Science
Research Mentor, NIH Minority Biomedical Research Support (MBRS) program at
SJSU
Collaborators and Other Affiliiations
Dr. Klaus Albert, Department of Chemistry, University of Tubingen, Tubingen,
Germany
Dr. Bogdan Buszewski, Department of Chemistry, Nicolas Copernicus University,
Torun, Poland
Dr. Milton Hearn, Professor, Biochemistry/Molecular Biology, Monash University,
Melbourne, Australia
Dr. Kiyokatsu Jinno, Department of Materials Science, Toyohashi, University,
Toyohashi, Japan
Dr. Antoine Siouffi, Department of Chemistry, Universite d'Aix-Marseille,
Marseille, France
Dr. Frantisek Svec, Co-Editor, Department of Chemistry, University of California, Berkeley
Graduate Advisor
Dr. James L. Sudmeier, Tufts University Medical School
Postdoctoral Advisor
Dr. Robert L. Pecsok, University of Hawaii (Retired)
Postgraduate Scholar Sponsor
Dr. Eric J. Williamsen (Camille and Henry Dreyfus Fellow), Ursinus College,
Collegeville Pennsylvania
Dr. G. Brent Dawson (Camille and Henry Dreyfus Fellow), University of North
Carolina, Greensboro
TOTAL NUMBER OF GRADUATE (M.S.) STUDENTS ADVISED: 75
TOTAL NUMBER OF POSTDOCTORAL SCHOLARS SPONSORED: 8
RECENT GRANTS
42. W.M.Keck Foundation, 2000-2002 $500,000
"Support of Departmental Equipment and Renovation
43. National Institutes of Health, 2000-2004 $220,921
"Evaluation of Microseparation Techniques
44. National Science Foundation, 2001-2004 $310,000
"Fabrication, Characterization and Applications of New Separation
Media
47. National Science Foundation, 2001-2004, "SJSU/IBM GOALI Program $750,000
TOTAL NUMBER OF GRANTS: 49